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KMID : 0352720000240010018
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Journal of Ginseng Research
2000 Volume.24 No. 1 p.18 ~ p.22
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Ginsenosides Inhibit N-, p-, arid Q-types but not L-type of Ca2+Channel in Bovine Chromaffin cells
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Seung Yeol Nah/Seok Choi
Se Yeon Jung/Hyun Oh Kim/Hack Seang Kim/Hye Whon Rhim/Seok Chang Kim/Seung Yeol Nah
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Abstract
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In previous reports we have shown that ginsenosides inhibit high threshold voltage-dependent
C
a
2
+
channels in neuronal cells. However, these studies did not show whether ginsenosides-induced inhibition of
C
a
2
+
currents discriminates among the various
C
a
2
+
channel subtypes, although it is known that there are at least five different
C
a
2
+
channel subtypes in neuronal cells. In this study we investigated the effect of ginsenosides on high threshold voltage-dependent
C
a
2
+
channel subtypes using their selective
C
a
2
+
channel blockers nimodipine (L-type),
ω
-conotoxin GVIA (N-type), or
ω
-agatoxin IVA (P-type) in bovine chromaffin cells. We could observe that ginsenosides inhibited high threshold voltage-dependent
C
a
2
+
currents in a dose-dependent manner. The
I
C
50
/ was about 120
μ
g/ml. Nimodipine had no effect on ginsenosides response. However, the effect of ginsenosides on
C
a
2
+
currents was reduced by
ω
-conotoxin GVIA,
ω
-agatoxin IVA, and mixture of nimodipine,
ω
-contoxin GVIA, and
ω
-agatoxin IVA. These data suggest that ginsenosides are negatively coupled to three types of calcium channels in bovine chromaffin cell, including an
ω
-conotoxin GVIA-sensitive (N-type) channel, an
ω
-agatoxin IVA-sensitive (P-type) channel and nimodipine/
ω
-conotoxin GVIA/
ω
-agatoxin IVA-resistant (presumptive Q-type) channel.Q-type) channel.
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KEYWORD
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Ginsenosides, Ca2+ channel subtypes, chromaffin cells, anti-stress,
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